Articles published in 2021

13 Aprile 2021
DOI: 10.17470/NF-021-0031

Effects of the cooking of mushrooms (Cantharellus symoensii; firifiti) on vitamin C, phenolic compounds and antioxidant activity

Ndlovu TV, Reid T, Chipurura B
Thermal treatments may lead to the degradation of phytochemical compounds in foods of plant origin. The selection of cooking methods is key to preventing nutritional losses. Therefore, the aim of this research was to determine the effects of different cooking methods on vitamin C, phenolic compounds and antioxidant activity in Cantharellus symoensii (firifiti). Frying significantly (p≤0.05) decreased vitamin C content, but significantly (p≤0.05) increased total phenolic content, flavonoid content and antioxidant activity. Boiling significantly (p≤0.05) decreased vitamin C, total phenolic content, flavonoid content and antioxidant activity. Microwaving resulted in a significant (p≤0.05) increase in the total phenolic content and antioxidant activity, but significantly (p≤0.05) reduced vitamin C and flavonoid content. Baking significantly (p≤0.05) decreased vitamin C and flavonoid content, but significantly (p≤0.05) increased the total phenolic content. All of the cooking methods decreased the vitamin C content. The order of DPPH activity was: fried > baked > microwaved > raw > boiled. From the four cooking methods studied, frying was established as being the most effective cooking method for retaining or enhancing mushroom bioactive compounds.
15 Marzo 2021
DOI: 10.17470/NF-021-0030

In vitro evaluation of the cytotoxicity of Nutri-pepper enhancer in human-like kidney and hepatic cells and mouse fibroblasts

Ann YC, Jam S, Tuah L
The aim of this paper was to evaluate the cytotoxicity of Nutri-pepper enhancer in fibroblasts, hepatic and kidney cells by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results showed no significant cytotoxic effects of Nutri-pepper enhancer on fibroblasts, hepatic and kidney cells at a concentration range of 0.9375–60 μg/ml. The IC50 values of the sample against the tested cell lines ranged between 5.53–14.39 μg/ml, which are much higher than those for the positive control with the IC50 values ranging between 0.0238–0.1179 μg/ml. This finding indicates that Nutri-pepper enhancer is not cytotoxic to fibroblast, kidney and hepatic cells.
29 Gennaio 2021
DOI: 10.17470/NF-021-0029

Use of a highly standardized mixture of Vitis vinifera, Ginkgo biloba and Melilotus officinalis extracts in the treatment of cellulite: a biopharmaceutical approach

Bertuccioli A, Bressan A, Biagi A, Neri M, Zonzini G
Cellulite currently remains a controversial topic to be defined and fully explained. The genesis of cellulite has a highly complex pathophysiology, which includes expansion of the subcutaneous adipose tissue, fibrotic dermal septa formation, progressive skin laxity, atrophy and structural modification of some dermal components. These alterations could also be caused by insufficiency of the precapillary sphincters affected by the development of cellulite. The study here encompassed a retrospective clinical trial that analyzed the results of 90 days of treatment in 21 adult women aged between 24 and 53 who underwent a motor programme for the treatment of cellulite along with using two different food supplements. The first was based on extracts of Vitis vinifera, Ginkgo biloba and Melilotus officinalis, with two subgroups at a dosage of two and three tablets per day, respectively; the second was based on carnosine and beta-alanine, constituting a control group. The weight, height, waist, hip, proximal thigh, mid-thigh, distal thigh and calf circumferences were measured for all subjects. There were no appreciable variations with respect to weight, BMI, waist circumference or the waist-to-hip ratio. The consumption of the mixture of Vitis vinifera, Ginkgo biloba and Melilotus officinalis extracts was associated with a greater reduction in hip circumference and thigh circumferences with a dosage of two tablets/day compared to three tablets/day, and compared to the control. The sample size yielded interesting but still not completely unequivocal results. Further studies with a greater number of subjects will be required to clarify the extent of the results obtained, while investigating the posology to determine the most effective treatment within the timeframe considered.