In vitro evaluation of the cytotoxicity of Nutri-pepper enhancer in human-like kidney and hepatic cells and mouse fibroblasts
The aim of this paper was to evaluate the cytotoxicity of Nutri-pepper enhancer in fibroblasts, hepatic and kidney cells by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results showed no significant cytotoxic effects of Nutri-pepper enhancer on fibroblasts, hepatic and kidney cells at a concentration range of 0.9375–60 μg/ml. The IC50 values of the sample against the tested cell lines ranged between 5.53–14.39 μg/ml, which are much higher than those for the positive control with the IC50 values ranging between 0.0238–0.1179 μg/ml. This finding indicates that Nutri-pepper enhancer is not cytotoxic to fibroblast, kidney and hepatic cells.
DOI: 10.17470/NF-021-0030
DATE: 2021
AUTHOR/S: Ann YC, Jam S, Tuah L
ABSTRACT:
The aim of this paper was to evaluate the cytotoxicity of Nutri-pepper enhancer in fibroblasts, hepatic and kidney cells by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results showed no significant cytotoxic effects of Nutri-pepper enhancer on fibroblasts, hepatic and kidney cells at a concentration range of 0.9375–60 μg/ml. The IC50 values of the sample against the tested cell lines ranged between 5.53–14.39 μg/ml, which are much higher than those for the positive control with the IC50 values ranging between 0.0238–0.1179 μg/ml. This finding indicates that Nutri-pepper enhancer is not cytotoxic to fibroblast, kidney and hepatic cells.
KEYWORDS:
Cytotoxicity, Nutri-pepper enhancer, MTT assay, IC50
DOI 10.17470/NF-021-0030